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Aiming at genetically modifying T cells with designed CAR, our scientists flexibly adopt versatile methodologies to transfect CAR construction into T cells, such as packing and transfection with virus based transfection methods. Since the successful manufacture of CAR-T cells is largely dependent on T cell receptor (TCR) gene transfer, this genetic modification of T lymphocytes is one of the most critical steps to generate superior CAR-T cells. Although the T cells are difficult to modify using non-viral methods like lipid-based car t plasmid transfection due to the high toxicity and low efficiency, the electroporation transfection and transposon based transfection systems are much more robust to complete the T-cell transfection. CAR-T cell transfection https://www.creative-biolabs.com/car-t/gene-packaging-delivery.htm