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The transmembrane region is the region of the protein sequence that spans the cell membrane. It is usually an α-helical structure with about 20-25 amino acid residues. Most of the amino acids that constitute the transmembrane protein are hydrophobic amino acids. It is very necessary to predict the transmembrane region of the sequence. Generally, membrane proteins cannot be expressed in a prokaryotic expression system. If the transmembrane region is predicted for the sequence and there is a transmembrane region in the sequence, then a eukaryotic expression system needs to be selected for expression in the follow-up; if you want to use a prokaryotic expression system for expression, you need Knock out the transmembrane region sequence. Now, the computational biologist of CD BioSciences is proud to tell you that we are willing to provide you with transmembrane prediction services! Transmembrane Prediction
https://www.computabio.com/transmembrane-prediction-services.html