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EC-D100T E.coli Host Cell DNA Residue Detection kit

The E.coli Host Cell DNA Residue Detection Kit can be used for Quantitative analysis of DNA residue in recombinant protein expressed products, purified intermediate and finished products from the host cell.

Specifications of E.coli Host Cell DNA Residue Detection kit
Introduction

This kit adopts Taqman probe fluorescence qPCR method. The kit has the advantages of high specificity and sensitivity by using specific primers & probes, LOQ can reach 30fg/μL level. The preparation process of DNA Control is completely consistent with National Standards, therefore it has high purity and no protein and ion interference. DNA Control has been calibrated by National Standards to ensure the accuracy of the sample quantitative detection. The kit provides a DNA Dilution Buffer, which enables good replicate parallelism in a single experiment and good reproducibility between multiple experiments. Purchasing this kit comes with a complimentary Magnetic Residual DNA Sample Preparation Kit-100T, designed for isolating residual DNA: CG-DP100.

Criteria for Results
Standard Curve: R ²> 0.99; Amplification Efficiency: 90% ≤ Eff% ≤ 110%; Slope: -3.8~-3.1.

The recovery rate of spiked samples=(measured value of spiked samples - measured value of samples)/theoretical value of spiked samples * 100%, with a range of 50% -150%.

No Template Control (NTC): In the reaction system, replacing the target template with DNA Dilution Buffer while keeping other components unchanged, and the Ct value obtained should be 'Undetermined' or Ct value≥35.

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