DIY - zrób to sam

Seamless Cloning Kit
https://www.hanbiology.com/products/seamless-cloning-kit/


Seamless cloning technique from HANBIO is a new, fast and simple cloning method, which can insert one or more target DNA fragments at any site of the plasmid without any restriction enzymes and ligases. Breaking through the traditional double enzyme digestion and linking, the recombinant vector of high efficiency cloning can be obtained by one-step recombinant method, which greatly improves the working efficiency.

Introduction of Seamless Cloning Kit
The HB-infusion™ seamless cloning kit developed by HANBIO is extremely simple to operate. It only needs to linearize the cloning site of the vector and introduce 15-25 bp homologous sequences that are completely consistent with both ends of the vector cloning site at the 5 'end of the insert PCR primer. The linearized cloning vector and the PCR fragments with homologous sequences were mixed in appropriate proportions and then added into HB-infusion™ Master mix. The directed cloning could be completed quickly by the reaction of DNA exonuclease, DNA polymerase and ligase in the reaction system at 50℃ for 20 minutes. The positive rate was almost 100%.