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BlueGene Biotech
HH-H0019-1 HEK 293 Host Cell Protein ELISA Kit, G2-96T
https://www.elisakit.cc/products/hh-h0019-1-hek-293-host-cell-protein-elisa-kit-96t/


BlueGene Biotech
HH-H0019-1 HEK293 Host Cell Protein Assay Kit, G2-96T
HEK 293 Host Cell Protein ELISA Kit, G2 is for research use only, it should not be used in clinical diagnostic procedures. The kit is intended for the quantitative determination of residual host cell protein in Cell Culture Supernatants, Protein Purification Process, and End-Product expressed by HEK 293 expression systems.

Specifications Of HH-H0019-1 HEK293 HCP ELISA Kit, G2


Principle of the Assay

This ELISA kit is applied to the solid phase sandwich Enzyme-Linked Immunosorbent Assay. The microplate has been pre-coated with a capture antibody. The antigen to be measured and the HRP-conjugated detection antibody are then added to the microplate wells sequentially to form a capture antibody-antigen-detection antibody complex. After washing, the conjugates uninvolved in the reaction are removed, and then a TMB substrate solution is added to the wells to incubate. TMB substrate solution turns blue after the oxidation of HRP and finally turns yellow immediately after adding the stop solution. The color of the TMB substrate positively correlated with the antigen bound in the initial steps. Measure the absorbance (OD value) at 450nm using a microplate reader, and create a standard curve along with the corresponding concentrations. Then, by inputting the OD values of the samples into the standard curve equation, calculate the concentration of the target protein in the sample.

Quality Control On HH-H0019-1 HEK HCP ELISA Kit, G2

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